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Registros recuperados : 15 | |
1. | | ALVAREZ, R. H.; MARTINEZ, A. C.; CARVALHO, J. B. P. de; ARCARO, J. R. P.; PIRES, R. M. L.; OLIVEIRA, C. A. de. Eficácia do tratamento Ovsynch associado à inseminação artificial prefixada em rebanhos Bos taurus e Bos indicus. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 2, p. 317-323, fev. 2003 Título em inglês: Efficacy of the Ovsynch treatment associated to fixed-time artificial insemination in Bos taurus and Bos indicus herds. Biblioteca(s): Embrapa Unidades Centrais. |
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2. | | CASTELANI, L.; PIZAURO, L. J. L.; MUSSI, F. L.; MOTTA, T. P.; POZZI, C. R.; ARCARO, J. R. P.; ZAFALON, L. F. Perfil de resistência antimicrobiana e produção de biofilme por Staphylococcus aureus isolados de novilhas. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26, 2011, Foz do Iguaçu, 2011. Anais... Foz do Iguaçu: SBM, 2011 Biblioteca(s): Embrapa Pecuária Sudeste. |
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4. | | LARA, M. A. C; RIBEIRO, J. A.; REICHERT, R. H.; PIRES, R. M. L.; ARCARO, J. R. P.; CARVALHO, J. B. P.; DEMARCHI, J. J. A. A.; MÉO, S. C.; BUFARAH, G.; SERENO, J. R. B.; SANTOS, S. A.; ABREU, U. G. P.; MIRANDA-SANTOS, I. K. F.; CONTEL, E. P. B. Relaciones genéticas entre razas bovinas con empleo de marcadores genéticos. In: SIMPÓSIO IBEROAMERICANO SOBRE CONSERVACIÓN Y UTILIZACIÓN DE RECURSOS ZOOGENÉTICOS, 6., 2005, Chiapas, México. Anais. Chiapas, México: CYTED: Universidad Autónoma de Chiapas, 2005. p.159-163. Biblioteca(s): Embrapa Pantanal. |
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5. | | MARTINS, T.; ROSA, A. F.; CASTELANI, L.; MIRANDA, M. S. de; ARCARO, J. R. P.; POZZI, C. R. Intramammary treatment with gentamicin in lactating cows with clinical and subclinical mastitis. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 4, p. 283-289, abr. 2016. Biblioteca(s): Embrapa Unidades Centrais. |
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6. | | MOTTA, T. P.; FRIZZARIN, A.; MARTINS, T.; MIRANDA, M. S.; ARCARO, J. R. P.; AMBRÓSIO, L. A.; POZZI, C. R. Estudo sobre a ocorrência de fungos e aflatoxina B1 na dieta de bovinos leiteiros em São Paulo. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 1, p. 23-28, jan. 2015. Título em inglês: Study on the occurrence of fungi and aflatoxina B1 in the diet of dairy cattle in São Paulo, Brazil. Biblioteca(s): Embrapa Unidades Centrais. |
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7. | | PILON, L. E.; ARCARO, J. R. P.; ZAFALON, L. F.; SANTANA, R. C. M. Identificação de staphylococcus coagulase negativo isolados de glândulas mamárias de novilhas tratadas no pré-parto. Identification of coagulase-negative staphylococci isolated from mammary glands of heifers treated pre-calving In: SIMPÓSIO INTERNACIONAL DE MEDICINA VETERINÁRIA PREVENTIVA, 2013, Jaboticabal. Novas tecnologias, novos desafios- anais. Ars Veterinária, v. 29, supl.1, 2013. Biblioteca(s): Embrapa Pecuária Sudeste. |
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8. | | PILON, L. E.; ARCARO, J. R. P.; ZAFALON, L. F.; SANTANA, R. C. M. Identification of coagulase-negative staphylococci isolated from mammary glands of heifers treated pre-calving. In: SIMPÓSIO INTERNACIONAL DE MEDICINA VETERINÁRIA PREVENTIVA, 2013, Jaboticabal. Novas tecnologias, novos desafios- anais. Ars Veterinária, v. 29, supl.1, 2013. Biblioteca(s): Embrapa Pecuária Sudeste. |
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9. | | ZAFALON, L. F.; ARCARO, J. R. P.; NADER FILHO, A.; FERREIRA, L. M.; CASTELANI, L.; BENVENUTTO, F. Investigação de perfis de resistência aos antimicrobianos em Staphylococcus aureus isolados na ordenha de vacas em lactação. Revista Instituto Adolfo Lutz, v. 67, n. 2, p. 118-125, 2008. Biblioteca(s): Embrapa Pecuária Sudeste. |
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10. | | ZAFALON, L. F.; ARCARO, J. R. P.; NADER FILHO, A.; FERREIRA, L. M.; CASTELANI, L.; BENVENUTTO, F. Diagnóstico laboratorial de staphylococcus aureus envolvidos na epidemiologia da mastite bovina. In: CONGRESSO BRASILEIRO DE QUALIDADE DO LEITE: SEGURANÇA ALIMENTAR E SAÚDE PÚBLICA, 3., 2008, Recife - anais. Recife:CBQL: UFRPE, 2008. 1 CD- ROM. Biblioteca(s): Embrapa Pecuária Sudeste. |
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15. | | ZAFALON, L. F.; POZZI, C. R.; CAMPOS, F. P.; ARCARO, J. R. P.; SARMENTO, P.; MATARAZZO, S. V. Boas práticas de ordenha. São Carlos, SP: Embrapa Pecuária Sudeste, 2008. 50 p. (Embrapa Pecuária Sudeste, Documentos, 78). Biblioteca(s): Embrapa Pecuária Sudeste. |
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Registros recuperados : 15 | |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
04/04/2017 |
Data da última atualização: |
10/07/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CLEMENTE, Z.; MARTINEZ, D. S. T.; OLIVEIRA, C. R. M.; MAURER-MORELLI, C. V.; NUNES, M. C. S.; CASTRO, V. L. S. S. de. |
Afiliação: |
Z. CLEMENTE; D. S. T. MARTINEZ; C. R. M. OLIVEIRA; C. V. MAURER-MORELLI; M. C. S. NUNES; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA. |
Título: |
Zebrafish exposure to graphene oxide is related to behavior changes. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: WORKSHOP IN ENVIRONMENTAL NANOTECHNOLOGY, 2., 2016. Book of Abstracts... Sorocaba: Unesp, 2016. |
Páginas: |
p. 27. |
Idioma: |
Inglês |
Conteúdo: |
Graphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the aim of this study was to investigate the implications of GO exposure in zebrafish behavior under environmental conditions. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. Twelve larvae per group were individually allocated in a 96 well-plate containing clean water and acclimated during 30 min. The following behavior?s biomarkers were monitored during 30 min through DanioVision video-tracking system: total time in movement, distance moved and swimming velocity. At the end of the experiment, larvae were fixed with PFA 4% and its total length and yolk sac were measured through a stereomicroscope. The mortality in all groups was below 10% and no malformation was observed. The statistical analysis (Two-way ANOVA followed by Bonferroni test) showed that, independently of HA presence, larvae exposed to GO dispended more time in movement, swam a greater distance, and showed higher swimming velocity than controls. The larvae exposed to GO, with or without HA, were about 3.8% shorter than control. There was no difference among groups related to yolk sac. The effect can be related to GO adsorption to larvae body that can act as tactile stimuli. Our previous study supports the hypothesis that GO can cause impairment in neuromuscular development. Further experiments are being performed to clarify these observations. MenosGraphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the aim of this study was to investigate the implications of GO exposure in zebrafish behavior under environmental conditions. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. Twelve larvae per group were individually allocated in a 96 well-plate containing clean water and acclimated during 30 min. The following behavior?s biomarkers were monitored during 30 min through DanioVision video-tracking system: total time in movement, distance moved and swimming velocity. At the end of the experiment, larvae were fixed with PFA 4% and its total length an... Mostrar Tudo |
Palavras-Chave: |
Nanotoxicology. |
Thesaurus NAL: |
Ecotoxicology; Embryotoxicity; Nanomaterials. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161628/1/clemente-zebrafish.pdf
|
Marc: |
LEADER 02991nam a2200229 a 4500 001 2068076 005 2017-07-10 008 2016 bl uuuu u00u1 u #d 100 1 $aCLEMENTE, Z. 245 $aZebrafish exposure to graphene oxide is related to behavior changes.$h[electronic resource] 260 $aIn: WORKSHOP IN ENVIRONMENTAL NANOTECHNOLOGY, 2., 2016. Book of Abstracts... Sorocaba: Unesp$c2016 300 $ap. 27. 520 $aGraphene oxide (GO) has been explored to application in areas such as pharmaceutical and environmental sciences. However, GO safety is still unclear. Beyond that, environmental factors, as the presence of humic substances in aquatic environment, can affect nanomaterials behavior and its toxicological effects. The motor development of zebrafish embryo has been strongly characterized and established to neurotoxicity studies. A previous study of our group revealed that the exposure of zebrafish embryos to GO is related to a reduction in total body length and in acetylcholinesterase activity. Morphological and biochemical changes can be related to behavior abnormalities and affect organism?s survival, leading to serious environmental consequences. Thus, the aim of this study was to investigate the implications of GO exposure in zebrafish behavior under environmental conditions. Zebrafish embryos (n=48/group) were exposed to GO (100 mg/L) in the presence or absence of humic acid (HA, 20 mg/L) during 5 days. Larvae were not feed during the experiment and were exposed under a 14/10 h light/dark cycle, 26 ± 1oC. Twelve larvae per group were individually allocated in a 96 well-plate containing clean water and acclimated during 30 min. The following behavior?s biomarkers were monitored during 30 min through DanioVision video-tracking system: total time in movement, distance moved and swimming velocity. At the end of the experiment, larvae were fixed with PFA 4% and its total length and yolk sac were measured through a stereomicroscope. The mortality in all groups was below 10% and no malformation was observed. The statistical analysis (Two-way ANOVA followed by Bonferroni test) showed that, independently of HA presence, larvae exposed to GO dispended more time in movement, swam a greater distance, and showed higher swimming velocity than controls. The larvae exposed to GO, with or without HA, were about 3.8% shorter than control. There was no difference among groups related to yolk sac. The effect can be related to GO adsorption to larvae body that can act as tactile stimuli. Our previous study supports the hypothesis that GO can cause impairment in neuromuscular development. Further experiments are being performed to clarify these observations. 650 $aEcotoxicology 650 $aEmbryotoxicity 650 $aNanomaterials 653 $aNanotoxicology 700 1 $aMARTINEZ, D. S. T. 700 1 $aOLIVEIRA, C. R. M. 700 1 $aMAURER-MORELLI, C. V. 700 1 $aNUNES, M. C. S. 700 1 $aCASTRO, V. L. S. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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1. | | 5150289, TENNESSEE FARM AND HOME SCIENCE, University of Tennessee. Agricultural Experiment Station, Knoxville-TN Biblioteca(s): Catálogo Coletivo de Periódicos Embrapa; Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Meio-Norte. | |
Registros recuperados : 1 | |
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